Download e-book for kindle: CRYO-EM, PART B: 3-D RECONSTRUCTION by Grant Jensen

By Grant Jensen

ISBN-10: 0123849918

ISBN-13: 9780123849915

This quantity is devoted to an outline of the tools, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the tips, intrumentation, and techniques underlying all cryo-EM techniques which enable practictioners to simply movement among them. inside of every one part, the articles are ordered based on the most typical symmetry of the pattern to which their equipment are applied.* Includes time-tested core methods and new innovations applicable to any researcher * equipment integrated are worthwhile to either established researchers and newbies to the sector * correct historical past and reference info given for systems can be utilized as a consultant

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Additional info for CRYO-EM, PART B: 3-D RECONSTRUCTION

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1970b). In this case, the solution to the problem of interpolation in Fourier space becomes a solution to the reconstruction problem. Crowther et al. (1970b) solved it as an overdetermined system of linear equations and gave the following least-squares solution: À Á ^ hkl ¼ WT W À1 WT D: D ð1:6Þ where D and Dhkl are written as 1D arrays D and Dhkl, respectively, and W denotes the appropriately dimensioned matrix of the interpolants in Eq. 5). For general cases the above method is impractical because of the large size of the matrix W.

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CRYO-EM, PART B: 3-D RECONSTRUCTION by Grant Jensen


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