By Melvin I. Simon
This quantity of equipment in Enzymology covers the present method for the detection and review of constitutively lively proteins. The chapters written through professional authors who're leaders within the field, provide tricks and methods now not on hand in basic study publications.It is broadly referenced, with helpful figures and tables through the volume.A. specialist authors who're leaders within the box B. broadly referenced and priceless figures and tables C. offers tricks and tips to facilitate replica of tools
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Extra resources for Constitutive Activity in Receptors and Other Proteins, Part 1
Gently maneuver the rat onto the fixed arm by inserting the arm into the rat’s ear. Still holding the rat steadily, slide the free arm into the rat’s other ear and tighten. Slide the nose piece back until the rat’s head is bent downward such that the top of the nose is perpendicular to the bench. Using a number 10 blade, make a skin incision approximately 2 cm in length down the midline beginning slightly higher than ear level. Using the same number 10 blade and holding the scalpel nearly horizontal, make an incision through the exposed muscle down the midline by resting the blade on the midline and applying a gentle rocking pressure.
Constructing a Chung board can be done easily with a piece of 1/4 in. pressboard cut to approximately 18 in. lengthwise and 6 in. wide. Cut three strips of heavy grade tubing (at least a 1/4 in. thick for strength, but still malleable by hand) to approximately 15 in. and tightly tie widthwise around the board to form three taught loops. Cut the excess tubing after a tight knot has been fashioned. ) 1 in. diameter rubber bands to the tubing (one band per tubing loop) by looping the band around the tubing and pulling through itself.
5. Parenteral injections in rats Parenteral injections in rats are similar to those in mice with the exception of concentration and volume. Parenteral injections in rats should have drug concentrations based on a 1 mL/kg volume. m. c. p injections. 5 in. needle fitted to a 1 cc syringe parallel to the body of the animal into the base of the fold formed when pinching skin on the dorsal thoracic area and gently pulling the skin away from the rat’s body. In general, no more than 1 mL may be administered subcutaneously to an adult rat.
Constitutive Activity in Receptors and Other Proteins, Part 1 by Melvin I. Simon