By Annette M. Griffin, Hugh G. Griffin
Institute of meals learn, Norwich, U.K. equipment in Molecular Biology sequence, quantity 24. First of a two-part sensible reduction to the researcher who makes use of desktops for the purchase, garage, or research of nucleic acid or protein sequences. Plastic comb binding. eleven participants, three U.S.
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Additional resources for Computer Analysis of Sequence Data Part 1 (Methods in Molecular Biology)
Selection of the Enzymes The two recommended replies to the question asked are either “*” for selecting all enzymes, or “**” for selecting all enzymes, including the isoschizomers. ” will explain the available options. 4. Definition of the Translation Scheme In this method, all three forward reading frames are displayed. To achieve three-letter translation, enter “T” (letter T in upper case). 5. map. 6. Review the Output The output is shown partially in Fig. 1. Note the coincidence of several cleavage sites, already mentioned in Methods 1 and 2.
1. Start of the Program map To start the program map, type the command map on the command line with the appropriate options to preselect enzymes (see Chapter 3, Method 1 per these options). Additionally, use the options silent and nocomp, and noscal in order to suppressthe sequencecomplement and the scale line. 2. Definition of Beginning and End This method requires that you know the beginning and ending coordinates of your reading frames. Therefore, the default values should be disregarded, and the numbers should be entered properly.
All GCG programs generally require a terminal that is capable of emulating the VT100 terminal mode standard. Scroll-back facilities as provided on workstations or in PC terminal emulators are advantageous but not essential. The text output can be reprocessed on word processing programs on personal computers. The data transfer capabilities required for this are usually provided within the terminal emulation program. The output form of the map program was designed by Schroeder and Blattner (2). If text output is to be printed, any ASCII code printer is sufficient.
Computer Analysis of Sequence Data Part 1 (Methods in Molecular Biology) by Annette M. Griffin, Hugh G. Griffin