By Antonia Vlahou, Manousos Makridakis
This moment editionexpands upon the former version with present, specified advancements within the box and brings jointly a multi-disciplinary workforce of prime researchers to supply their newest protocols for scientific proteomics research. Written within the hugely winning Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and tips about troubleshooting and averting identified pitfalls.
Authoritative and sensible, Clinical Proteomics: tools and Protocols, moment Edition offers methods that may function a competent consultant to researchers, together with clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators engaged on biomarker development.
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Extra resources for Clinical Proteomics: Methods and Protocols
Aust N Z J Obstet Gynaecol 47:9–15 Pereira L, Reddy AP, Jacob T et al (2007) Identification of novel protein biomarkers of preterm birth in human cervical-vaginal fluid. J Proteome Res 6:1269–1276 Body Fluids-SOPs 84. Davalieva K, Kiprijanovska S, Noveski P et al (2012) Human seminal plasma proteome study: a search for male infertility biomarkers. Balkan J Med Genet 15:35–38 85. Milardi D, Grande G, Vincenzoni F et al (2012) Proteomic approach in the identification of fertility pattern in seminal plasma of fertile men.
Serum-supplemented growth medium: DMEM (Dulbecco’s modified Eagle medium), High Glucose, GlutaMAX™, Pyruvate, supplemented with 10 % (v/v) FBS and 1 % (v/v) Penicillin/Streptomycin (P/S) (see Note 1). Store at 4 °C (see Note 2). 2. Phosphate-Buffered Saline: 1× in sterile Ultra Pure Water. 34 Agnieszka Latosinska et al. Fig. 3 Comparative analysis of identified proteins from secretome (classical and non-conventional approach) and total cell extract. (I) Comparison of all identified proteins. Venn diagrams representative of all identified proteins from conditioned medium (a) and ER/Golgi fraction (b).
Husi H, Van Agtmael T, Mullen W et al (2014) Proteome-based systems biology analysis of the diabetic mouse aorta reveals major changes in fatty acid biosynthesis as potential hallmark in diabetes associated vascular disease. Circ Cardiovasc Genet 7(2):161–170 23. Deutsch EW, Mendoza L, Shteynberg D et al (2010) A guided tour of the trans-proteomic pipeline. Proteomics 10:1150–1159 41 24. Carty DM, Siwy J, Brennand JE et al (2011) Urinary proteomics for prediction of preeclampsia. Hypertension 57:561–569 25.
Clinical Proteomics: Methods and Protocols by Antonia Vlahou, Manousos Makridakis