By Adam E. Golan
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Extra info for Cellulase : types and action, mechanism, and uses
2007]. 9. CLONING AND EXPRESSION OF CELLULASE GENES Recombinant DNA techniques offer powerful means to solve various problems which arise both in the development of efficient cellulose producers for commercial applications and in the studies of complex cellulolytic microbial systems. , 1991]. Moreover, the expression of protein fused with suitable tags permits the separation of the chimeras also when they are expressed in a cellulolytic host. Cellulase genes have been cloned from different microbial genera into various suitable hosts.
Morana, L. Maurelli, E. Ionata et al. used as carbon source for Penicillium funiculosum growth. The best results, in terms of cellulolytic enzymes production, were observed when sugar cane bagasse was treated with acid and subsequently, alkali in order to obtain partially delignified cellulignin. The culture filtrate of P. funiculosum contained several cellulolytic activities. 9. Cellulases from P. , 2010]. Two new fungal strains from subtropical soils, Penicillium sp. CR-316 and Penicillium sp.
The only activity exhibited by Cel61A was the endoglucanase activity toward substrates containing 1,4-β-glycosidic bonds (CMC, hydroxyethylcellulose and β-glucan). Table 5. , 2004 In recent years, research on Trichoderma has been facilitated significantly by sequencing of the genomes of three strains representing the most important applications of this genus. The genome of T. , 2008]. Analyses and annotation of the genomes of T. atroviride and T. jgipsf. html), are still in progress. The Fungus Acremonium cellulolyticus, isolated in 1987, is known to be a potent producer of cellulases as T.
Cellulase : types and action, mechanism, and uses by Adam E. Golan