By Christopher D. Richardson
Baculovirus Expression Protocols deals either commercial and university-based researchers a finished comilation of the most recent baculovirus thoughts in addition to step by step directions and time-saving innovations. The contributors-leading specialists within the field-present the varied expression plasmids presently in use, consultant the reader throughout the technique of producing and choosing recombinant virus, and describe particular examples of recombinant protein creation and purification. The emphasis is on substitute and easier screening options for the choice of recombinant baculovirus. The publication additionally surveys a few of the insect telephone traces presently appropriate with the baculovirus procedure. Highlights contain construction of recombinant virus utilizing linearized DNA and vectors that comprise a b-galactosidase indicator and an entire record of expression vectors at present to be had. precise descriptions for the scale-up of protein creation utilizing spinner flasks, bioreactors, and bug larvae also are integrated. Baculovirus Expression Protocols bargains either business and university-based researchers a very good selection of reproducible, step by step laboratory protocols. it is going to instantly turn into critical for a person operating with baculoviruses and their software within the expression of recombinant proteins in insect cells.
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Additional info for Baculovirus Expression Protocols
ZacZ) to ease the selection of recombinant viruses in the plaque assays subsequent to cotransfection. Transfer vectors analogous to pAcUW3, capable of synthesizing two experimental gene products, have not been reported in the literature. One such vector was developed in our laboratory. This has been designated pAcUW41 and is marketed by PharMingen. It consists of a copy of the polyhedrin gene promoter inserted in tandem with the p10 gene promoter. The transcripts initiating at the p10 promoter are terminated by SV40 signals inserted between the two promoters.
Such vectors are probably now becoming redundant with the advent of the polymerase chain reaction, which can be used to quickly tailor a foreign gene to incorporate an ATG codon. One feature of these vectors that may still be useful, is that some foreign proteins synthesized as a fusion product are more stable in baculovirus-infected cells. If the recombinant gene product is only required for the production of antibody, the additional sequences may not present a problem. Recently, some vectors that produce fusion proteins with the S-glutathione transferase have been developed.
If the recombinant gene product is only required for the production of antibody, the additional sequences may not present a problem. Recently, some vectors that produce fusion proteins with the S-glutathione transferase have been developed. In these vectors, a protease cleavage site is present at the junction of the fused protein, allowing the recovery of an almost intact protein (46,47; R. F. Weaver and M. Lopez-Ferber, unpublished data [pAcRW4]). , thrombin) used, some GST residues may remain attached to the cleaved recombinant protein.
Baculovirus Expression Protocols by Christopher D. Richardson