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Two examples are _-amylase of Bacillus stearothermophilus (96) and cellulase of the alkaliphilic Bacillus BCE103 (97). Amylases have been used for many years to remove starch sizes from fabrics, known as desizing. Originally, amylases from plant or animal sources were used. Later, they were replaced by amylases of bacterial origin. The first bacterial enzyme for desizing was _-amylase from Bacillus subtilis, which was commercialized in the early 1950s (96). A novel _-amylase naturally produced by B.
One is the segregational instability of recombinant plasmids, which results in the loss of recombinant plasmids and therefore loss of the desired traits. For example, expression of the Bacillus thuringiensis (Bt) toxin gene from a high copy number vector in Pseudomonas fluorescens was undetectable because of plasmid instability (47). Segregational instability of plasmids is usually resolved by maintaining recombinant strains under selective pressures, usually by means of antibiotics. However, concerns about the use, release, and horizontal transfer of antibiotic resistance marker genes suggest that other means of maintaining plasmid stability need to be developed.
In 2003, an industrial working grouping in Canada established a new and rigorous set of guidelines for assessing the cost and benefits of introducing a new GMO in the supply chain. 2. Several US industry groups, including the Grocery Manufacturing and Food Marketing Institute, enforced stricter regulation of biopharma compliance. 3. The US National Organic Program has imposed limits on GMOs. Public opposition in Europe appears to be hardening. Europe plans to require labeling of products derived from GMOs (54).
Amsacrine-Associated Cardiotoxicity by dr.salem