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Ageing Dev. 98, 1–35. 77. Pignolo, R. , Masoro, E. , Nichols, W. , Brandt, C. , and Cristofalo, V. J. (1992) Skin fibroblasts from aged Fischer 344 rats undergo similar changes in replicative life span but not immortalization with caloric restriction of donors. Exp. Cell Res. 201, 16–22. Fibroblast Model for Cell Senescence Studies 49 78. Bruce, S. A. and Deamond, S. F. (1991) Longitudinal study of in vivo wound repair and in vitro cellular senescence of dermal fibroblasts. Exp. Gerontol. 26, 17–27.

And Kritchevsky, D. (1965) Growth and glycolysis in the human diploid cell strain WI-38. Proc. Soc. Exp. Biol. Med. 118, 1109–1113. 131. Levine, E. M. (1972) Mycoplasma contamination of animal cell cultures: a simple, rapid detection method. Exp. Cell Res. 74, 99–109. Human T-Cell Clones 53 3 Human T-Cell Clones Graham Pawelec 1. Introduction Techniques for generating human T-cell clones (TCCs) were first described nearly two decades ago (1,2). This was a direct consequence of the discovery of T-cell growth factor and the subsequent ability to propagate T-cells over extended periods (3).

1969) Diabetes mellitus and aging: diminished plating efficiency of cultured human fibroblasts. Proc. Natl. Acad. Sci. USA 64, 155–160. 72. Martin, G. M. (1978) Genetic syndromes in man with potential relevance to pathobiology of aging, in Genetic Effects on Aging (Bergsma, D. and Harrison, D. ), Alan Liss, New York, pp. 5–39. 73. Danes, B. S. (1971) Progeria: a cell culture study on aging. J. Clin. Invest. 50, 2000–2003. 74. Schneider, E. L. and Epstein, C. J. (1972) Replication rate and lifespan of cultured fibroblasts in Down’s syndrome.

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Aging Methods and Protocols (Methods in Molecular Medicine) by Yvonne A. Barnett

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